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KMID : 0371019970300020369
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Journal of Preventive Medicine and Public Health
1997 Volume.30 No. 2 p.369 ~ p.380
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Effects of Mercury Chloride on Nitric Oxide Syntheses in Mouse Peritoneal Macrophage and EMT-6 Cell
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Kwon Keun-Sang
Koh Dai-Ha
Ki No-Suk
Youm Jung-Ho
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Abstract
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The effects of treatment with mercury chloride on the nitrite, 4nd nitrate syntheses were observed in peritoneal macrophages from Balb/c mice and MT-6 cells in vitro. The cells were cultured in Dulbecco´s modified Eagle´s medium(t)MEM) with cytokines.
Amounts of nitrite and nitrate in the culture media after 24 and 36 hours of culture were about 2-fold, and 3-fold of those measured after 12 hours´, respectively. There were very close associations between the amounts of nitrite and ~´ nitrate measured in the culture media according to culture time.
The survival rate of peritonea] macrophages was significantly decreased by mercury chloride added into the media in dose-dependent manner, however the survivals of EMT-6 cells were not influenced by mercury chloride concentratAo, n in media. Nitrite and nitrate syntheses were dose-dependently decreased by mercur chloride added in culture media. ATP synthesis also decreased in EMT-6 cells by mercury chloride.
These results reported here suggest that the disorder of cell mediated immunity by mercurials could be related to the inhibition of nitric oxide synthesis which seems to be caused by the inhibition of ATP synthesis.
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KEYWORD
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Nitrite, Nitrate, ATP, Macrophage, EMT-6 cell, Mercury
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